Abstract
DURING the course of experiments designed to examine the effects of inhalation anaesthetics on the sodium, potassium and water contents of isolated rat atrial tissue1, it was noted that the standard deviations of the sodium values were rather high. These values were thought to be unreliable and were therefore not reported. It was felt that part of the deviations in sodium values could be explained by the fact that uniform blotting procedures were not used on all tissues, thus removing variable quantities of sodium-rich bathing medium. Part of the difference could also be explained on the basis of contamination of the tissue or its digest from any number of sources, despite diligent care to use demineralized water and clean apparatus. Since the tissue was so small (about 50 mg) and its sodium content so small, it is easy to see that the slightest contamination would give erroneous results. As the experiments progressed, however, and greater attention was directed to the possibilities of contamination, the sodium values decreased and the standard deviations also decreased.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Paradise, R. R., and Griffith, L. K., Anesthesiology, 25, 106 (1964).
Paradise, R. R., Nature, 198, 112 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PARADISE, R., MATTHEWS, W. & ROESCH, R. Effect of Tissue Size on Measurements of Sodium, Potassium and Water Contents of Isolated Rat Ventricular Tissue. Nature 205, 82 (1965). https://doi.org/10.1038/205082a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/205082a0
This article is cited by
-
Functional acclimation of Japanese quail to simulated high-altitude
Journal of Comparative Physiology ? B (1974)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.