Abstract
MEL has recently reviewed the sedimentation properties of nucleated and non-nucleated cells in normal rat bone marrow1. The complex nature of the factors that are involved in the separation of marrow cells by sedimentation technique are emphasized in his comprehensive paper. Tsuji reported a method for separation of guinea-pig bone marrow cells by using layers of gum acacia solutions of varying densities2. Myeloid cells were obtained in practically pure state. Gradient techniques involving several kinds of solutions, such as sucrose and bovine plasma albumin, have been used by others3.
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References
Mel, H. C., Nature, 200, 423 (1963).
Tsuji, Kenzo, Osaka City Med. J., 8, 91 (1962).
Goodman, J. W., Exp. Cell Research, 21, 88 (1960).
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ARCHDEACON, J., GLAZEBROOK, G. & WISE, W. Simple Separation of Rabbit Marrow Myeloid Cells from Erythroid Cells. Nature 204, 996 (1964). https://doi.org/10.1038/204996a0
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DOI: https://doi.org/10.1038/204996a0
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