Abstract
GLUCOSAMINE and galactosamine are usually identified by the ninhydrin degradation method devised by Gardell, Heijkenskjöld and Roch-Norlund1, and Stoffyn and Jeanloz2. The method suffers, however, from the disadvantage that glucosamine and mannosamine are not distinguishable under these conditions, since the two hexosamines are converted into the same pentose, that is, arabinose. This communication describes a new method which permits the identification of the three isomers of the hexosamines.
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Gardell, S., Heijkenskjöld, F., and Roch-Norlund, A., Acta Chem. Scand., 4, 970 (1950).
Stoffyn, P. J., and Jeanloz, R. W., Arch. Biochem. Biophys., 52, 373 (1954).
Ohkuma, S., and Shinohara, T., Proc. Japan Acad., 39, 686 (1963).
Partridge, S. M., Biochem. J., 42, 238 (1948).
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OHKUMA, S., SHINOHARA, T. Identification of Hexosamines by Two-dimensional Paper Electrophoresis and Chromatography. Nature 202, 593 (1964). https://doi.org/10.1038/202593a0
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DOI: https://doi.org/10.1038/202593a0
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