Abstract
SINCE 1959, when Singer1 succeeded in conjugating antibody globulin with ferritin, an electron-dense label, this technique has been utilized by several workers to localize various antigens by electron microscopy (cited in ref. 2). Singer used m-xylylene diisocyanate (XC) and later toluene-2,4-diisocyanate (TC)3 as conjugation agents. With both XC and TC the conjugation procedure is carried out in a two-stage reaction. In the first stage ferritin reacts with the coupling agent. In the second reaction the XC- (TC-) modified ferritin is conjugated with the antibody globulin. More recently Tawde and Ram4 have described a one-step method using p, p′-difluro-m, m′-dinitrodiphenyl sulphone (FNPS) as coupling reagent in which there is little loss in the precipitating capacity of antibody.
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References
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VOGT, A., KOPP, R. Loss of Specific Agglutinating Activity of Purified Ferritin-conjugated Antibodies. Nature 202, 1350–1351 (1964). https://doi.org/10.1038/2021350a0
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DOI: https://doi.org/10.1038/2021350a0
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