Abstract
FERRITIN prepared from horse-spleen by Granick's method1 consists of a mixture of a colourless component and a heterogeneous coloured material2. The coloured iron-containing material, ferritin, sediments with a widely spread boundary and is separated by starch-gel and polyacrylamide-gel electrophoresis into at least three distinct fractions (α-, β- and γ-ferritin, that is, bands 1, 2 and 3)3–6. The colourless iron-free component moves with a sharp boundary in the ultracentrifuge {S020,w = 17.6; mol. wt. 465,000) and is homogeneous in starch-gel and polyacrylamide-gel5. From ultracentri-fugal investigations Rothen2 concluded that apoferritin obtained by reduction of ferritin with sodium dithionite7 was identical with the colourless component which he called ‘free’ apoferritin.
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References
Granick, S., J. Biol. Chem., 146, 451 (1942).
Rothen, A., J. Biol. Chem., 152, 679 (1944).
Theron, J. J., Hawtrey, A. O., and Schirren, V., Clin. Chim. Acta, 8, 165 (1963).
Kopp, R., Vogt, A., and Maass, G., Nature, 198, 892 (1963).
Kopp, R., Vogt, A., and Maass, G., in Protides of the Biological Fluids, Proc. eleventh Colloq., Bruges, 1963, edit. by Peeters, H. (Elsevier, Amsterdam, 1964).
Richter, G. W., Brit. J. Exp. Path., 45, 88 (1964).
Granick, S., and Michaelis, L., J. Biol. Chem., 147, 91 (1943).
Behrens, M., and Taubert, M., Z. physiol. Chem., 290, 156 (1952).
Unpublished.
Wieme, R. J., Studies on Agar Gel Electrophoresis (Arscia Uitgaven N.V., Brussels, 1959).
Smithies, O., Biochem. J., 61, 629 (1955).
Ouchterlony, Ö., in Progress in Allergy, 6 (1962).
Schultze, H. E., Haupt, H., Heide, K., and Heimburger, N., Clin. Chim. Acta, 8, 207 (1963).
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KOPP, R., VOGT, A. & MAASS, G. ‘Free’ Apoferritin and Apoferritin obtained by Reduction of Iron-containing Ferritin. Nature 202, 1211–1212 (1964). https://doi.org/10.1038/2021211b0
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DOI: https://doi.org/10.1038/2021211b0
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