Abstract
IN cell culture investigations, the degree of ‘conditioning’ of the medium during growth is frequently of crucial importance1,2. With the usual techniques of monolayer cell culture which use multiple Petri dishes, Leighton tubes, French square and prescription bottles, etc., small variations in pH, temperature, cell inoculum size, degree of attachment and growth of cells, volume of medium delivered, degree of evaporation of medium, etc., are compounded so that the degree of ‘conditioning’ frequently varies from preparation to preparation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cox, R. P., and Pontecorvo, G., Proc. U.S. Nat. Acad. Sci., 47, 839 (1961).
Cox, R. P., and MacLeod, C. M., Proc. U.S. Nat. Acad. Sci., 49, 504 (1963).
Oyama, V. I., and Eagle, H., Proc. Soc. Exp. Biol. and Med., 91, 305 (1956).
Fredricsson, B., Anat. Anz., 99, 97 (1952).
Fredricsson, B., Acta Anat., 26, 246 (1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MARTIN, G. Culture of Replicate Monolayers of Fibroblasts under Identical Conditions. Nature 201, 1338–1340 (1964). https://doi.org/10.1038/2011338b0
Issue Date:
DOI: https://doi.org/10.1038/2011338b0
This article is cited by
-
A simple device for planting replicate mammalian cell cultures
In Vitro (1972)
-
A simple device for planting replicate mammalian cell cultures
In Vitro (1972)
-
Mitotic Nondisjunction in Cultivated Human Cells
Nature (1967)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
