Abstract
IN 1916 Rous and Jones1 used trypsin to disperse avian and mammalian cells. Since then some relatively specific proteases, for example, elastase2 and collagenase3, have been used, but trypsinization has remained the standard method for this purpose. Trypsin has many drawbacks. Digestion is slow and incomplete, the cells tend to remain in clumps and there is a viscous residue.
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References
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GWATKIN, R., THOMSON, J. A New Method for Dispersing the Cells of Mammalian Tissues. Nature 201, 1242–1243 (1964). https://doi.org/10.1038/2011242b0
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DOI: https://doi.org/10.1038/2011242b0
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