Abstract
IT has been shown earlier that actinomycin D inhibits the multiplication of influenza viruses without any influence on Newcastle disease virus and Sendai virus multiplication1,2. It was found that the dynamics of Sendai virus reproduction in actinomycin-treated cultures of chicken fibroblasts and human amniotic cells were changed after the latent period of multiplication was shortened by 2 h (Fig. 1). To clarify this problem an autoradiographic investigation was carried out. Cultures of human amniotic cells infected with Sendai virus (1,000 ID50 per cell) were held at 35° C in Medium 199 or in this medium with 0.5 γ/ml. of actinomycin D. 30 min, 1, 2, 3 and 5 h after onset of incubation samples of cultures were exposed to 1 µc/ml. of tritiated uridine for 30 min, washed in BBS containing excess of unlabelled uridine, fixed in Carnoy solution, covered with emulsion type M and exposed for 1 month. Developed autographs were stained by the Unna method.
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References
Barry, R. D., Ives, D. R., and Cruickshank, J. G., Nature, 194, 1139 (1962).
Zhdanov, V. M., and Bukrinskaya, A. G., Probl. of Virol., 2, 230 (1963).
Perry, R. P., Exp. Cell Res., 29, 400 (1963).
Perry, R. P., Proc. U.S. Nat. Acad. Sci., 48, 12, 2179 (1962).
Bukrinskaya, A. G., Zhdanov, V. M., and Ramenskaya, G. P., Probl. of Virol., 6, 547 (1961).
Zhdanov, V. M., Bukrinskaya, A. G., and Ramenskaya, G. P., Acta virol., 7, 1, 1 (1963).
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BUKRINSKAYA, A., ZHDANOV, V. Shortening by Actinomycin D of Latent Period of Multiplication of Sendai Virus. Nature 200, 920–921 (1963). https://doi.org/10.1038/200920a0
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DOI: https://doi.org/10.1038/200920a0
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