Abstract
IN an investigation of conditions which produce in vitro changes of myelin similar to those which occur in Wallerian degeneration (with formation of ovoids and spheres) the importance of the presence of a sufficient amount of calcium in the medium became apparent. A similar observation was made years ago by Nageotte1. The effects of high concentrations of calcium or of sodium ions on the constitution of myelin were therefore examined. Central and peripheral myelins homogenized with equal amounts of either 1 M sodium chloride or 0.5 M calcium chloride were extracted by water or by chloroform/methanol (2 : 1) and the extracts and residues were chemically analysed. Comparable experiments were performed by histochemical means on teased and cryostat-sectioned fresh central and peripheral nervous tissue.
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WOLMAN, M., WIENER, H. Changes in the Constitution of Myelin caused by Ions. Nature 200, 886–887 (1963). https://doi.org/10.1038/200886a0
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DOI: https://doi.org/10.1038/200886a0
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