Abstract
IN the course of an investigation into the cause of death in intestinal obstruction in dogs, we have examined material obtained from dogs with isolated loops prepared by the technique of Harper and Blain1. Such material is known to be toxic when injected intravenously in small quantities into healthy dogs2–4. In attempting to define the nature of the toxic substance, we have examined its effect on isolated smooth muscle. Peritoneal fluid and loop contents from some dogs caused slow contractions of smooth muscle which were not abolished by atropine, mepyramine or lysergic acid diethylamide. Material was diluted 10–100 times and was applied directly to a guinea pig ileum preparation at 35° C. Contractions were slow in onset and reached a maximum in about 1 min. On changing the fluid in the tissue bath the muscle relaxed within 1–2 min. Similar, but less potent, activity was also shown by intestinal contents of normal dogs.
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WEIPERS, W., SANFORD, J. Demonstration of Kinins in Intestinal Obstruction in Dogs. Nature 198, 813 (1963). https://doi.org/10.1038/198813a0
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DOI: https://doi.org/10.1038/198813a0
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