Purification of Psittacosis Agent with Anion Exchange Cellulose–‘ECTEOLA’

Abstract

IN our investigations of the 6BC(7 strain of psittacosis it became necessary to obtain large quantities of highly purified suspensions of organisms with a high infectivity titre. At first we used a line of the 6BC strain adapted to growth in the allantoic cavity of embryonated eggs from which the organisms were extracted by differential centrifugation and trypsinization. The yield of organisms was always small and the final preparations were heavily contaminated with host material. The use of fluoro-carbon (‘Genetron 113’) according to the method of Gessler et a1.¹ resulted in almost complete loss of infectivity.