Abstract
THE Michaelis–Menten1 equation relating initial velocity of a simple enzyme-catalysed reaction to initial substrate concentration is: where ν is the initial velocity of reaction, e is the initial enzyme concentration, s is the initial substrate concentration, k3 is the velocity constant of the breakdown of the enzyme-substrate compound into enzyme and product of the catalysed reaction and K is the substrate concentration at which ν is half-maximal.
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Michaelis, L., and Menten, M. L., Biochem. Z., 49, 333 (1913).
Briggs, G. E., and Haldane, J. B. S., Biochem. J., 19, 338 (1925).
Warburg, O., and Christian, W., Biochem. Z., 298, 150 (1938).
Long, C., Biochemists' Handbook, 362 (E. and F. N. Spon, Ltd., London, 1961).
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FISHER, R. Significance of the Michaelis Constant. Nature 197, 799 (1963). https://doi.org/10.1038/197799a0
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DOI: https://doi.org/10.1038/197799a0
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