Abstract
FOR prolonged storage of biological materials it is generally considered that temperatures of −100° C. or lower are necessary1, and as thermodynamic changes have been demonstrated in mixtures of glycerol and Earle's solution in this temperature region2 and in ice at −130° C.3 it is preferable to use a lower storage temperature such as is provided by liquid nitrogen.
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NAGINGTON, J., GREAVES, R. Preservation of Tissue Culture Cells with Liquid Nitrogen. Nature 194, 993–994 (1962). https://doi.org/10.1038/194993b0
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DOI: https://doi.org/10.1038/194993b0
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