Abstract
A NOVEL method for the fluorescent labelling of proteins has been described recently which involved treatment of a protein solution with an active labelling dye dispersed, on diatomaceous earth or other inert materials1. This modification afforded a considerable saving in time and minimized protein denaturation as compared with earlier techniques. The experiments reported here constitute a further improvement of this procedure which obviates time-consuming dialysis and permits fluorescent labelling of protein solutions, removal of hydrolysed or unreacted dye and change of buffer as well as pH in less than 30 min.
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References
Rinderknecht, H., Experientia, 16, 430 (1960).
Riggs, J. L., Loh, P. C., and Eveland, W. C., Proc. Soc. Exp. Biol. Med., 105, 655 (1960).
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RINDERKNECHT, H. Ultra-Rapid Fluorescent Labelling of Proteins. Nature 193, 167–168 (1962). https://doi.org/10.1038/193167b0
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DOI: https://doi.org/10.1038/193167b0
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