Abstract
WEIMER et al.1 developed the process used mostly to isolate seromucoid acid from sera. But the salting out with ammonium sulphate results in the protein precipitate appearing with 60–70 per cent sulphate and this is carried away. While performing experiments on precipitation and denaturation of serum proteins by phenol with high concentrations of salt2, I noticed that seromucoid acid was present in the supernatant. A new process was therefore devised and perfected, combining the elimination of the proteins by phenol, with the help of sodium chloride and fractionation by ammonium sulphate.
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References
Weimer, H. E., Mehl, J. W., and Winzler, R. J., J. Biol. Chem., 185, 569 (1950).
Michon, J. (unpublished results, 1961).
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MICHON, J. Preparation of Orosomucoid (α1-Acid Seromucoid). Nature 193, 1077–1078 (1962). https://doi.org/10.1038/1931077a0
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DOI: https://doi.org/10.1038/1931077a0
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