Abstract
THE isolation of dihydrosphingosine from cerebrosides of beef brain and spinal cord by Carter et al. 1 raised questions regarding its distribution in different sphingolipids and its metabolic relationships to sphingosine. However, no method for the routine differentiation of sphingosine and dihydrosphingosine appeared until very recently with the gas chromatographic method of Sweeley and Moscatelli2. Wittenberg3 separated the N-succinyl derivatives of sphingosine and related compounds by reversed phase chromatography on diatomaceous earth treated with dichlorodimethylsilane, but he was unable to separate sphingosine and dihydrosphingosine. This communication describes a reversed-phase paper chromatographic method for the separation of the dinitrophenyl derivatives of sphingosine and dihydrosphingosine.
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References
Carter, H. E., Norris, W. P., Glick, F. J., Phillips, G. E., and Harris, R., J. Biol. Chem., 170, 269 (1947).
Sweeley, C. C., and Moscatelli, E. A., J. Lipid Res., 1, 40 (1959).
Wittenberg, J. B., J. Biol. Chem., 216, 379 (1955).
Carter, H. E., Nalbandov, O., and Tavormina, P. A., J. Biol. Chem., 192, 197 (1951).
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KARLSSON, KA. Separation of Sphingosine and Dihydrosphingosine by Reversed Phase Paper Chromatography. Nature 188, 312–313 (1960). https://doi.org/10.1038/188312a0
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DOI: https://doi.org/10.1038/188312a0
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