Abstract
THE function of soluble ribonucleic acid in the transfer of activated amino-acids to the microsomal ribonucleoprotein particles has been correlated to the presence of a specific terminal −pCpCpA group1–3, and the occurrence of pseudouridylic acid in the molecules4. One of the basic requirements for the full understanding of the structural basis of the function of soluble ribonucleic acid would be the preparation of a ribonucleic acid sample which is homogeneous, unaltered, and having a functional activity not different from that present in the living cells. The principal purpose of this study has been to establish the technique whereby a sample of acid which satisfies the above requirements is prepared. The attempt towards this direction led to preparation of an active ribonucleic acid sample which is homogeneous when judged by electrophoretic examination and sedimentation analyses.
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OTAKA, E., OSAWA, S. Preparation and Some Properties of a Soluble Ribonucleic Acid in Yeast. Nature 185, 921–922 (1960). https://doi.org/10.1038/185921a0
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DOI: https://doi.org/10.1038/185921a0
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