Serum Chromatography an Columns connected to a Common Source of Buffer Gradients

Abstract

RECENTLY there has been much progress in the separation of serum proteins by column chromatography. The best resolution of the protein components is theoretically obtained by the application of buffer and salt gradients to the column¹. This principle has been applied during the elution of human serum from modified cellulose anion exchange columns when large numbers of chromatographic peaks were observed². However, this technique is time-consuming, and it is difficult to produce an identical sequence of buffer gradients for each experiment. A modification using a single buffer gradient has been described for the separation of serum components of clinical interest³, but this method reveals fewer components than the original technique. To overcome these difficulties a method with high resolving power for the simultaneous comparison of sera using the same system of buffer gradients has been developed and investigated.