Abstract
DURING the investigation of a new histochemical substrate, Nα-benzoyl-dl-arginine-β-naphthylamide liydrochloride, localization by a simultaneous azo coupling reaction of enzymatic activity to mast cells in human and dog tissues was noted. Recently, Benditt has described the presence of an enzyme in the mast cells of numerous species resembling bovine α-chymotrypsin. This enzyme hydrolyses the histochemical substrate 3-chloroacetyl-2-naphthoic acid anilide1. Although Nα-benzoyl-dl-arginine-β-naphthylamide hydrochloride has been shown in biochemical systems to be a substrate for trypsin and not for α-chymotrypsin, carboxypeptidase, liver esterase or ‘leucine’ aminopeptidase2, it was thought of importance to attempt to define as closely as possible the characteristics of the enzyme in mast cells hydrolysing Nαa - benzoyl - dl - arginine - β - naphthylamide hydrochloride and to differentiate it from other known mast cell enzymes.
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References
Benditt, E., and Arase, M., J. Exp. Med., 110, 451 (1959).
Riedel, A., and Wünsch, E., Hoppe-Seylers Z. physiol. Chem., 316, 61 (1959).
Holland, G. F., and Cohen, L. A., J. Amer. Chem. Soc., 80, 3765 (1958).
After this synthesis had been completed, preparation of Nα-benzoyl-dl-arginme-β-naphthylamide hydrochloride via the carbodiimide procedure was reported (cf. ref. 2).
Gomori, G., J. Histochem. Cytochem., 1, 469 (1953).
Fast corinth V from General Dyestuffs Co., New York.
Fast garnet GBC from General Dyestuffs Co., New York.
Fast blue B from General Dyestuffs Co., New York. Michrome blue 603 (diazotized 4′-amino-2′,5′-dimethoxybenzanilide), from Edward Gurr, Ltd., London, may also be used.
Benditt, E., and Arase, M., J. Histochem. Cytochem., 6, 431 (1958).
Braun-Falco, O., Nature, 183, 51 (1959).
Hill, M., Nature, 180, 654 (1957).
Ungar, G., and Damgaard, E., J. Exp. Med., 101, 1 (1955).
Snellman, O., Sylvén, B., and Julén, C., Biochim. Biophys. Acta, 7, 98 (1951).
Keller, R., Int. Arch. Allergy, 11, 328 (1957).
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GLENNER, G., COHEN, L. Histochemical Demonstration of a Species-Specific Trypsin-like Enzyme in Mast Cells. Nature 185, 846–847 (1960). https://doi.org/10.1038/185846a0
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DOI: https://doi.org/10.1038/185846a0
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