Abstract
THE contamination of tissue culture cell lines by pleuropneumortia-like organisms has become an increasingly important problem1–4. The contamination itself is insidious and cannot be detected ordinarily by gross changes in the appearance of the media macroscopically or microscopically. The tissue culture cells themselves, when observed microscopically, may or may not exhibit slight cytopathology as evidenced by intracytoplasmic granularity. Properly stained preparations may reveal intracytoplasmic particles, presumably the contaminating pleuropneumonia-like organisms5–7.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Robinson, L. R., Wichelhausen, R. H., and Roizman, B., Science, 124, 1147 (1956).
Collier, L. H., Nature, 180, 757 (1957).
Rothblatt, G. O., and Morton, H. E., Bact. Proc., 73 (1958).
Hayflick, L., and Stinebring, W. R., paper presented at a Conference on the Biology of the Pleuropneumonia-like Organisms, Ann. N.Y. Acad. Sci., 79, 433 (1960).
Hayflick, L., and Stinebring, W. R., Anat. Rec., 121, 477 (1955).
Hayflick, L., Stinebring, W. R., Breckenridge, F. C., and Pomerat, C. M., Bact. Proc., 75 (1956).
Shepard, M. C., J. Bact., 75, 351 (1958).
Leberman, P. R., Smith, P. F., and Morton, H. E., J. Urol., 64, 167 (1950).
Leberman, P. R., Smith, P. F., and Morton, H. E., J. Urol., 68, 399 (1952).
Tulasne, R., Revue d'Immunologie, 15, 223 (1951).
Edward, D. G. ff., J. Gen. Microbiol., 10, 27 (1954).
Selawry, O. S., Goldstein, M. N., and McCormick, T., Cancer Res., 17, 785 (1957).
Hearn, jun., J. H., Officer, J. E., Elsner, V., and Brown, A., J. Bact., 78, 574 (1959).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HAYFLICK, L. Decontaminating Tissue Cultures infected with Pleuropneumonia-like Organisms. Nature 185, 783–784 (1960). https://doi.org/10.1038/185783a0
Issue Date:
DOI: https://doi.org/10.1038/185783a0
This article is cited by
-
Somatic cell hybrid selection with a transfectable dominant marker
Somatic Cell and Molecular Genetics (1984)
-
Elimination ofM. hyorhinis from murine neuroblastoma cell lines by in vivo passage
In Vitro (1982)
-
Lipid accumulation in cells derived from porcine aorta and grown under anaerobic conditions
Lipids (1976)
-
Elimination of mycoplasmas from cell cultures with sodium polyanethol sulphonate
Nature (1975)
-
Mycoplasma interactions with cell cultures, uncultured living cells and the problems posed by their presence in tissue cultures
Klinische Wochenschrift (1972)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
