Abstract
IN Roth and Schuster's method1 for colorimetric determination of tryptophan in vegetable matter, the measurement of the yellow colour is made with a Pulfrich Stufenfotometer with filter S 43. The method has often been used, but I have been unable to find any published account of the measurements made spectrophotometrically. I have therefore tested the method for some cereals, etc., by means of a Beckman spectrophotometer, model D.U.
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References
Roth, H., and Schuster, Ph., Angew. Chem., 52, 149 (1939).
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LARSEN, I. Spectrophotometric Determination of Tryptophan by the Method of Roth and Schuster: Some Sources of Error. Nature 185, 316–317 (1960). https://doi.org/10.1038/185316a0
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DOI: https://doi.org/10.1038/185316a0
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