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Mediation of the Genetic Control of Protein Synthesis in Cell-free Extracts of Neurospora crassa

Abstract

I HAVE recently demonstrated the development of tryptophan synthetase activity by cell-free extracts from conidia of wild-type Neurospora crassa. The process appeared to be one of synthesis of enzyme protein, and was associated with the net increase in the protein content of the system1. Similar extracts prepared from the td 2 tryptophan-less mutant strain neither contained nor developed any detectable tryptophan synthetase activity, but did catalyse a net synthesis of protein.

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References

  1. Wainwright, S. D., Canad. J. Biochem. Physiol. (in the press).

  2. Suskind, S. R., and Kurek, L. I., Science, 126, 1068 (1957).

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  3. Yanofsky, C., in “Methods in Enzymology”, 2, ed. by S. P. Colowick and N. O. Kaplan (Academic Press, Inc., New York, 1955).

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WAINWRIGHT, S. Mediation of the Genetic Control of Protein Synthesis in Cell-free Extracts of Neurospora crassa . Nature 185, 314–315 (1960). https://doi.org/10.1038/185314a0

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