Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Preparation of Crystalline Cytochrome553 from Porphyra tenera

Nature volume 185, pages 105–106 (1960)Cite this article

Abstract

PARTIAL purification of a cytochrome component from Porphyra tenera, a component which has absorption peaks similar to that of cytochrome c or c 1 was first reported by Yakushiji1. Recently, Katoh and Takamiya (ref. 2 and Katoh, S., and Takamiya, A., personal communication) succeeded in obtaining a crystalline preparation of this component by means of diethylaminoethyl-cellulose column chromatography and reported the redox-potential of this and other components in marine algae. We also have crystallized the cytochrome by means of a cation-exchanger at pH 5.0. Since the diethylaminoethyl-cellulose column procedure is possible at pH 7.0, this procedure may be better; our method can be used to purify not only basic enzyme proteins, but also those that are stable in acidic pH.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Yakushiji, E., Acta Phytochim., 8, 325 (1935).

    CAS  Google Scholar 

  2. Katoh, S., J. Biochem. (Japan), 46, 629 (1959).

    Article  Google Scholar 

  3. Kohn, J., Nature, 183, 1055 (1959).

    Article  ADS  CAS  Google Scholar 

  4. Horio, T., Higashi, T., Matsubara, H., Kusai, K., Nakai, M., and Okunuki, K., Biochim. Biophys. Acta, 29, 297 (1958).

    Article  CAS  Google Scholar 

  5. Okunuki, K., Sekuzu, I., Yonetani, T., and Takemori, S., J. Biochem. (Japan), 45, 847 (1958).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science, Toho University, Narashino, Chiba

    EIJIRO YAKUSHIJI & YASUTOMO SUGIMURA

  2. Department of Biology, Faculty of Science, University of Osaka, Osaka, Japan

    ICHIRO SEKUZU, ICHIRO MORIKAWA & KAZUO OKUNUKI

Authors
  1. EIJIRO YAKUSHIJI
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. YASUTOMO SUGIMURA
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. ICHIRO SEKUZU
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. ICHIRO MORIKAWA
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. KAZUO OKUNUKI
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

YAKUSHIJI, E., SUGIMURA, Y., SEKUZU, I. et al. Preparation of Crystalline Cytochrome553 from Porphyra tenera . Nature 185, 105–106 (1960). https://doi.org/10.1038/185105a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/185105a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing