Abstract
Vennes and Gerhardt1 demonstrated quite conclusively by serological means that no trace of cell-wall antigens remained attached to the protoplast membranes prepared by lysozyme treatment of Bacillus megaterium. Because of the differences in the chemical composition of walls of Gram-positive and Gram-negative bacteria it has been suggested that the osmotically fragile, spherical bodies produced from Gram-negative bacteria by lysozyme treatment or induced by penicillin action have only partially lost their cell-wall material2. Salton and Shafa3 have shown that some cell-wall components remain in spheroplasts prepared by the penicillin method from two Gram-negative species. Their results indicated that the lipo-protein components remained attached to the spheroplasts. The wall preparations from the spheroplasts were also agglutinated by a cell-wall antiserum.
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References
Vennes, J. W., and Gerhardt, Ph., J. BacteriOl., 77, 581 (1959).
Weibull, C., “Ann. Rev. Microbiol.”, 12, 1 (1958).
Salton, M. R. J., and Shafa, F., Nature, 181, 1321 (1958).
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Kabat, E. A., and Mayer, M. M., “Experimental Immunochemistry” (Charles C. Thomas, Springfield, Ill., 1948).
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HOLME, T., MALMBORG, AS. & COTA-ROBLES, E. Antigens of Spheroplast Membrane Preparations from Escherichia coli B. Nature 185, 57–58 (1960). https://doi.org/10.1038/185057b0
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DOI: https://doi.org/10.1038/185057b0
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