Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Occurrence of 3-Indolylacetic and 3-Indolecarboxylic Acids in Tomato Crown—Gall Tissue Extracts

Abstract

A NUMBER of publications1 report high auxin activity in extracts of tumour tissue initiated by the crown-gall organism, Agrobacterium tumefaciens, and indirect evidence indicates a high rate of auxin metabolism in this tissue2. Suggestions have been made that 3-indolylacetic acid is involved, although this has not been proved. There is evidence that auxin activity occurs at the same or a similar R F as 3-indolylacetic acid on one-dimensional chromatograms and other investigations indicate that 3-indolylacetic acid or “an auxin with same R F” is present in extracts of aseptically cultured gall tissue of tobacco and sunflower3. We have carried out single and two-dimensional paper chromatography with the acid fraction from ether extracts of large quantities of tomato crown-gall tissue grown on the stems of whole plants to determine whether 3-indolylacetic acid is in fact present.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Link, G. K. K., and Eggers, V., Bot. Gaz., 103, 87 (1941). Henderson J. H., and Bonner, J., Amer. J. Bot., 39, 444 (1952). Kulescha, Z., Année biol., 30, 319 (1954).

    Article  CAS  Google Scholar 

  2. Locke, S. B., Riker, A. J., and Duggar, B. M., J. Agric. Res., 57, 21 (1938).

    Google Scholar 

  3. Bitancourt, A. A., Année biol., 30, 361 (1954). Schwarz, K. R., Dierberger, R., and Bitancourt, A. A., Arch. Biol., S. Paulo, 22, 93 (1955).

    Google Scholar 

  4. Fawcett, C. H., Pascal, R. M., Pybus, M. B., Taylor, H. F., Wain, R. L., and Wightman, F., Proc. Roy. Soc., B, 150, 95 (1959).

    Article  ADS  CAS  Google Scholar 

  5. Kaper, J. M., and Veldstra, H., Biochim. Biophys. Acta, 30, 401 (1958). Schwarz, K., and Bitancourt, A. A., Science, 126, 607 (1957).

    Article  CAS  Google Scholar 

  6. Booth, A., J. Exp. Bot., 9, 306 (1958).

    Article  CAS  Google Scholar 

  7. Nichols, R., Nature, 181, 919 (1958).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

CLARKE, G., DYE, M. & WAIN, R. Occurrence of 3-Indolylacetic and 3-Indolecarboxylic Acids in Tomato Crown—Gall Tissue Extracts. Nature 184, 825–826 (1959). https://doi.org/10.1038/184825a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/184825a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing