Abstract
SUCCESSFUL cultures have recently been obtained from a stock strain of T. vaginalis which had been stored in a carbon dioxide cabinet for 26 months. The previous maximum survival reported was four months1. Since 1954, freezing in the presence of 5 per cent glycerol has been used for the maintenance of a strain of T. vaginalis. Stock cultures in the cabinet are replaced at approximately six-monthly intervals. Survival of cultures is shown on thawing by actively motile Protozoa and successful subcultures. The known maximum period of survival of such cultures has gradually been extended first to 8 months and then to 12 months.
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References
McEntegart, M. G., J. Hyg., 52, 545 (1954).
McEntegart, M. G., J. Clin. Path., 5, 275 (1952).
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McENTEGART, M. Prolonged Survival of Trichomonas vaginalis at −79° C.. Nature 183, 270–271 (1959). https://doi.org/10.1038/183270a0
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DOI: https://doi.org/10.1038/183270a0
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