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Bacteriophage Enrichment in a Liquid Two-Phase System with Subsequent Treatment with ‘Freon’ 113

Nature volume 183, pages 1070–1072 (1959)Cite this article

Abstract

THE possibility of concentrating protein molecules in one of the phases in a liquid two-phase system of polymers has been investigated by Albertsson1,2, who showed that the distribution coefficient can be related to the size of the molecules in accordance with the Brønsted3 formula. Albertsson and Frick4 have shown that in a dextran–methylcellulose system this relation holds for particles up to the size of phages, the upper limit at present being the size, or rather the surface area of T 2 phage. The concentration of T 2 in the bottom dextran-rich phase will be 400 times higher than in the methylcellulose-rich top phase if the two phases are allowed to separate for 48 hr. in the cold.

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Authors and Affiliations

  1. Institute of Biochemistry, Uppsala

    G. FRICK & P. Å. ALBERTSSON

Authors
  1. G. FRICK
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  2. P. Å. ALBERTSSON
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FRICK, G., ALBERTSSON, P. Bacteriophage Enrichment in a Liquid Two-Phase System with Subsequent Treatment with ‘Freon’ 113. Nature 183, 1070–1072 (1959). https://doi.org/10.1038/1831070a0

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