Abstract
WHEN a monolayer of chick fibroblasts is exposed to infection with a suitable dilution of Western Equine Encephalomyelitis virus and afterwards incubated under a layer of agar containing cell nutrients, areas of cell destruction or ‘plaques’ are produced; these were shown by Dulbecco1 to result from infection by single virus particles. The plaque technique has since been applied to a number of other viruses; but the presence of cell destruction or a cytopathic effect is normally considered to be a prerequisite for the success of the technique.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dulbecco, R., Proc. U.S. Nat. Acad. Sci., 38, 747 (1952).
Kissling, R. E., Proc. Soc. Exp. Biol. N.Y., 96, 290 (1957).
Bhatt, P. V., and Work, T. H., Proc. Soc. Exp. Biol. N.Y., 96, 213 (1957).
Porterfield, J. S. (unpublished results).
Theiler, M., and Smith, H. H., J. Exp. Med., 65, 767 (1937).
Gey, G. O., and Gey, M. K., Amer. J. Cancer, 27, 45 (1936).
Trans. Roy. Soc. Trop. Med. and Hyg. (to be published).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PORTERFIELD, J. Plaque Production with Yellow Fever and Related Arthropod-borne Viruses. Nature 183, 1069–1070 (1959). https://doi.org/10.1038/1831069b0
Issue Date:
DOI: https://doi.org/10.1038/1831069b0
This article is cited by
-
Characteristics of venezuelan equine encephalomyelitis virus ribonucleoprotein
Archiv f�r die gesamte Virusforschung (1971)
-
Formation of Infectious Arbovirus Ribonucleoprotein in Subcellular Structures
Nature (1970)
-
Study of the conditions of formation of interferon in a tissue culture of chick embryo fibroblasts infected with Japanese encephalitis virus
Bulletin of Experimental Biology and Medicine (1966)
-
Growth characteristics of Japanese encephalitis virus in chick embryo cells with special reference to the applicability of the culture to the production of inactivated vaccine
Archiv f�r die gesamte Virusforschung (1966)
-
A Plaque Technique for the Primary Isolation of Arthropod-borne Viruses
Nature (1960)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.