Abstract
WHILE the observation has been made that surface-active agents will produce lysis of the plasma membrane of cells1,2, little use seems to have been made of this observation for the preparation of nuclei3. Some preliminary observations suggest that nuclei may be prepared in large numbers from a variety of cells, without the use of special apparatus, by treatment with the anionic surface-active agent, sodium lauryl sulphate. Nuclei prepared in this manner are at least as free from cytoplasm as those prepared by the method of Hogeboom et al. 4. In the case of some tumours, such as the V–2 carcinoma of the rabbit, they are very much cleaner (Figs. 1 and 2).
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References
Bennett, L. R., and Connon, F. E., J. Nat. Cancer Inst., 19, 999 (1957).
Höber, R., and Höber, J., J. Gen. Physiol., 25, 705 (1942).
Dounce, A. L., and Lan, T. H., Science, 97, 584 (1943).
Hogeboom, G. H., Schneider, W. C., and Striebich, M. J., J. Biol. Chem., 196, 111 (1952).
Kay, E. R. M., Simmons, N. I., and Dounce, A. L., Fed. Proc., 10, 177 (1951) utilized 0.0142 M sodium lauryl sulphate to dissociate nucleic acid from protein.
Philpot, J. St. L., and Stainer, J. E., Biochem. J., 63, 214 (1956).
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HODES, M., PALMER, C. & WILLIAMS, D. Possible Use of Synthetic Surface-active Agents for the Preparation of Tumour Cell Nuclei. Nature 182, 529–530 (1958). https://doi.org/10.1038/182529a0
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DOI: https://doi.org/10.1038/182529a0
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