Abstract
DURING the past eight years of large-scale cultivation of Myc. tuberculosis var. bovis βAN5β on the Dorset-Henley synthetic liquid medium1,2 for the production of tuberculin purified protein derivative, from time to time and especially during the past two years we have met serious difficulties due to premature sinking of the pellicles, about 3β6 weeks after inoculation (the normal time before harvesting being 8 weeks). Growth came to a standstill and losses in tuberculin production were substantial.
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DEKKER, T., HUITEMA, H. Importance of Zinc for the Growth of Surface-Culture of Mycobacterium tuberculosis var. bovis and Large-scale Production of Tuberculin Purified Protein Derivative. Nature 182, 1387β1388 (1958). https://doi.org/10.1038/1821387a0
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DOI: https://doi.org/10.1038/1821387a0
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