Abstract
FAGREUS1 and others2,3 have shown that certain tissues from pre-sensitized animals can form antibody in vitro. This communication describes a technique whereby antibody production by single cells isolated in microdroplets can be detected. The technique is based on specific immobilization of Salmonella serotypes by anti-flagellar antibody. It was observed that single cells from a rat, simultaneously stimulated with two antigens, formed detectable amounts of one or the other antibody.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Fagreus, A., J. Immunol., 58, 1 (1948).
Thorbecke, G. J., and Keuning, F. J., J. Immunol., 70, 129 (1953).
Wesslen, T., Acta Dermato-Venerol., 32, 265 (1952).
Lederberg, J., Genetics, 41, 845 (1956).
Lederberg, J., J. Bacteriol., 68, 258 (1954).
Harris, S., Harris, T. N., and Farber, M. B., J. Immunol., 72, 148 (1954).
Da Fonbrune, P., “Technique de micromanipulation” (Masson, Paris 1949).
Burnet, F. M., Austral. J. Sci., 20, 67 (1957).
Talmage, D. W., “Ann. Rev. Med.”, 8, 239 (1957).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
NOSSAL, G., LEDERBERG, J. Antibody Production by Single Cells. Nature 181, 1419–1420 (1958). https://doi.org/10.1038/1811419a0
Issue Date:
DOI: https://doi.org/10.1038/1811419a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.