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Histochemical Detection with Ditetrazolium Chloride of some Enzymatic Activities in Isolated Mitochondria

Abstract

IN an earlier paper1, I have reported that ditetrazolium chloride, a substance introduced by Seligman and Ruthenburg2 for the histochemical detection of succinoxidase in tissue sections, can also be used for the detection of the same enzyme in mitochondria isolated from kidney and liver. This has been recently confirmed by Schiebler3 on the granula isolated from the neurohypophysis by differential sedimentation.

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References

  1. Dianzani, M. U., Exp. Cell Research (in the press).

  2. Seligman, A. M., and Ruthenburg, A. M., Science, 113, 317 (1951).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Schiebler, T. H., Z. Zellforsch., 36, 563 (1952).

    CAS  PubMed  Google Scholar 

  4. Schneider, W. C., J. Biol. Chem., 176, 259 (1948).

    CAS  PubMed  Google Scholar 

  5. Dianzani, M. U., Arch. Fisiologia, 50, 187 (1951).

    CAS  Google Scholar 

  6. Dianzani, M. U., Arch. Fisiologia, 50, 181 (1951).

    CAS  Google Scholar 

  7. Kennedy, E. P., and Lehninger, A. L., J. Biol. Chem., 179, 957 (1948); 185, 275 (1950).

    Google Scholar 

  8. Claude, A., Amer. Assoc. Adv. Sci. Research Conference on Cancer, Gibson Island, 1945.

  9. Dianzani, M. U., Arch. Sci. Biol., 34, 453 (1950).

    CAS  Google Scholar 

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DIANZANI, M. Histochemical Detection with Ditetrazolium Chloride of some Enzymatic Activities in Isolated Mitochondria. Nature 171, 125–126 (1953). https://doi.org/10.1038/171125b0

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