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Post-Transplant Events

Influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant

Bone Marrow Transplantation volume 39, pages 121–125 (2007)Cite this article

Abstract

Microbiological contamination of manipulated blood products, including hematopoietic progenitors obtained from peripheral blood, is an infrequent but persistent problem in transplant units. The relevance of such contamination in causing patient infection has been reported as insignificant, but the effect on the post-transplant course has not been well documented. We studied the incidence of bacterial contamination in autologous peripheral blood progenitor cell transplants in two of the bench processing steps, as well as the repercussions in the post-transplant course affecting incidence of infections, transfusion requirements and time to engraftment. A total of 365 aphereses performed on 152 patients were cryopreserved in 617 bags. In 31 of these bags (5.0%), bacterial cultures were positive for Coagulase-negative Staphylococcus (31.1%), S. epidermidis (21.9%), Corynebacterium sp. (6.3%), S. warneri (6.3%), Stenotrophomonas maltophilia (6.3%), Streptococcus sp. (9.4%), Viridans group Streptococcus (3.1%) and more than one bacteria (Coagulase-negative Staphylococcus plus Corynebacterium) (15.6%). Half of the bags were contaminated at the time of freezing and the others at the time of thawing. The 31 contaminated bags were infused into 17 patients. In five of these the same contaminating bacteria was found. No difference between the two groups of patients (contaminated and non-contaminated) was found on the day the fever started, length of fever, blood transfusion requirements and engraftment, but length of hospitalization was significantly greater in patients receiving contaminated transplants.

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References

  1. Kuehnert MJ, Roth VR, Haley NR, Gregory KR, Elder KV, Schreiber GB et al. Transfusion-transmitted bacterial infection in the United States, 1998 through 2000. Transfusion 2001; 41: 1493–1499.

    Article  CAS  Google Scholar 

  2. Jafari M, Forsberg J, Gilcher RO, Smith JW, Crutcher JM, McDermott M et al. Salmonella sepsis caused by a platelet transfusion from a donor with a pet snake. N Engl J Med 2002; 347: 1075–1078.

    Article  Google Scholar 

  3. Sazama K . Reports of 355 transfusion-associated deaths: 1976 through 1985. Transfusion 1990; 30: 583–590.

    Article  CAS  Google Scholar 

  4. Chang AH, Kirsch CM, Mobashery N, Johnson N, Levitt LJ . Streptococcus bovis septic shock due to contaminated transfused platelets. Am J Hematol 2004; 77: 282–286.

    Article  Google Scholar 

  5. Padley DJ, Greiner CW, Heddlesten TL, Hopkins MK, Maas ML, Gastineau DA . Endogenous microbial contamination of cultured autologous preparations in trials of cancer immunotherapy. Cytotherapy 2003; 5: 147–152.

    Article  CAS  Google Scholar 

  6. Webb IJ, Coral FS, Andersen JW, Elias AD, Finberg RW, Nadler LM et al. Sources and sequelae of bacterial contamination of hematopoietic stem cell components: implications for the safety of hematotherapy and graft engineering. Transfusion 1996; 36: 782–788.

    Article  CAS  Google Scholar 

  7. Attarian H, Bensinger WI, Buckner CD, McDonald DL, Rowley SD . Microbial contamination of peripheral blood stem cell collections. Bone Marrow Transplant 1996; 17: 699–702.

    CAS  PubMed  Google Scholar 

  8. Padley D, Koontz F, Trigg ME, Gingrich R, Strauss RG . Bacterial contamination rates following processing of bone marrow and peripheral blood progenitor cell preparations. Transfusion 1996; 36: 53–56.

    Article  CAS  Google Scholar 

  9. Schwella N, Rick O, Heuft HG, Miksits K, Zimmermann R, Zingsem J et al. Bacterial contamination of autologous bone marrow: reinfusion of culture-positive grafts does not result in clinical sequelae during the post-transplantation course. Vox Sang 1998; 74: 88–94.

    Article  CAS  Google Scholar 

  10. Prince HM, Page SR, Keating A, Saragosa RF, Vukovic NM, Imrie KR et al. Microbial contamination of harvested bone marrow and peripheral blood. Bone Marrow Transplant 1995; 15: 87–91.

    CAS  Google Scholar 

  11. Laura R, Degl'Innocenti M, Mocali M, Alberani F, Boschi S, Giraudi A et al. Comparison of two different time interval protocols for central venous catheter dressing in bone marrow transplant patients: results of a randomized, multicenter study. The Italian Nurse Bone Marrow Transplant Group (GITMO). Haematologica 2000; 85: 275–279.

    CAS  PubMed  Google Scholar 

  12. Espinosa MT, Fox R, Creger RJ, Lazarus HM . Microbiologic contamination of peripheral blood progenitor cells collected for hematopoietic cell transplantation. Transfusion 1996; 36: 789–793.

    Article  CAS  Google Scholar 

  13. Cohen A, Tepperberg M, Waters-Pick B, Coniglio D, Perfect J, Peters WP et al. The significance of microbial cultures of the hematopoietic support for patients receiving high-dose chemotherapy. J Hematother 1996; 5: 289–294.

    Article  CAS  Google Scholar 

  14. Kamble R, Pant S, Selby GB, Kharfan-Dabaja MA, Sethi S, Kratochvil K et al. Microbial contamination of hematopoietic progenitor cell grafts-incidence, clinical outcome, and cost-effectiveness: an analysis of 735 grafts. Transfusion 2005; 45: 874–878.

    Article  Google Scholar 

  15. Nasser RM, Hajjar I, Sandhaus LM, Hall GS, Avery RK, Bolwell BJ et al. Routine cultures of bone marrow and peripheral stem cell harvests: clinical impact, cost analysis, and review. Clin Infect Dis 1998; 27: 886–888.

    Article  CAS  Google Scholar 

  16. Rowley SD, Davis J, Dick J, Braine HG, Charache P, Saral R et al. Bacterial contamination of bone marrow grafts intended for autologous and allogeneic bone marrow transplantation. Incidence and clinical significance. Transfusion 1988; 28: 109–112.

    Article  CAS  Google Scholar 

  17. Jestice HK, Farrington M, Hunt C, Matthews I, Scott MA, Foreman J et al. Bacterial contamination of peripheral blood progenitor cells for transplantation. Transfus Med 1996; 6: 103–110.

    Article  CAS  Google Scholar 

  18. Nifong TP, Ehmann WC, Mierski JA, Domen RE, Rybka WB . Favorable outcome after infusion of coagulase-negative staphylococci-contaminated peripheral blood hematopoietic cells for autologous transplantation. Arch Pathol Lab Med 2003; 127: e19–e21.

    Google Scholar 

  19. Sugrue MW, Hutcheson CE, Fisk DD, Roberts CG, Mageed A, Wingard JR et al. The effect of overnight storage of leukapheresis stem cell products on cell viability, recovery, and cost. J Hematother 1998; 7: 431–436.

    Article  CAS  Google Scholar 

  20. Gorin NC . Collection, manipulation and freezing of haemopoietic stem cells. Clin Haematol 1986; 15: 19–48.

    Article  CAS  Google Scholar 

  21. Cassens U, Ahlke C, Garritsen H, Krakowitzky P, Wullenweber J, Fischer RJ et al. Processing of peripheral blood progenitor cell components in improved clean areas does not reduce the rate of microbial contamination. Transfusion 2002; 42: 10–17.

    Article  Google Scholar 

  22. Kipp F, Linnemann E, Fischer RJ, Sibrowski W, Cassens U . Cryopreservation reduces the concentration of detecTable bacteria in contaminated peripheral blood progenitor cell products. Transfusion 2004; 44: 1098–1103.

    Article  Google Scholar 

  23. Lazarus HM, Magalhaes-Silverman M, Fox RM, Creger RJ, Jacobs M . Contamination during in vitro processing of bone marrow for transplantation: clinical significance. Bone Marrow Transplant 1991; 7: 241–246.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Servicio de Hematologia, Hospital V. Arrixaca, Murcia, Spain

    M J Majado, A García-Hernández, A Morales, C González, V Martínez-Sánchez, P Rosique, A Rubio & E Sanz-Imedio

  2. Servicio de Microbiologia, Hospital V. Arrixaca, Murcia, Spain

    A Menasalvas

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  1. M J Majado
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Correspondence to M J Majado.

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Majado, M., García-Hernández, A., Morales, A. et al. Influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant. Bone Marrow Transplant 39, 121–125 (2007). https://doi.org/10.1038/sj.bmt.1705549

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  • DOI: https://doi.org/10.1038/sj.bmt.1705549

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