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Low CXCR4 membrane expression on CD34+ cells characterizes cells mobilized to blood

Bone Marrow Transplantation volume 37, pages 19–23 (2006)Cite this article

Summary:

In this work, association between the presence and membrane density of CXCR4 and the effectiveness of mobilization was studied. Ninety G-CSF mobilized PBPC and 28 native BM (nBM) preparations obtained from healthy individuals for transplantation and BM obtained after G-CSF mobilized PBPC collection in 10 donors were investigated. Positivity for CD34, HLA-DR and CXCR4 were analysed in the three colour fluorescence. The cellular profile of PBPC differed from nBM preparations with respect to lower: (i) proportion of CD34+ cells (0.64%±0.04 vs 0.92±0.07) and CD34+CXCR4+ cells (0.30%±0.02 vs 0.61±0.07); (ii) contribution of CXCR4+ cells to CD34+ cells (52.2%±2.5 vs 62.2%±4.2); (iii) CXCR4 epitope density in CD34+ cells (48.9±5.5 vs 94.7±10.4). PBPC yield for CD34+ cells was correlated with the content of CD34+ cells lacking CXCR4 in the leukapheresis product (R=0.38). In contrast, nBM harvested for transplantation was poor in CD34+ cells if these cells were frequently CXCR4− (R=−0.49). The present study shows that CD34+ cells mobilized to blood were characterized with a low proportion of CXCR4 and this associated with CD34+ cell content in PBPC.

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Acknowledgements

This work was supported in part by FP6 AlloStem project.

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  1. L. Hirszfeld Institute of Immunology and Experimental Therapy/Lower Silesian Centre for Cellular Transplantation and National Bone Marrow Donor Registry, Wroclaw, Poland

    D Dlubek, D Drabczak-Skrzypek & A Lange

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  1. D Dlubek
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Correspondence to A Lange.

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Dlubek, D., Drabczak-Skrzypek, D. & Lange, A. Low CXCR4 membrane expression on CD34+ cells characterizes cells mobilized to blood. Bone Marrow Transplant 37, 19–23 (2006). https://doi.org/10.1038/sj.bmt.1705198

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