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Allografting

Transplantation of CD34+ selected peripheral blood to HLA-identical sibling patients with aplastic anaemia: results from a single institution

Bone Marrow Transplantation volume 36, pages 325–329 (2005)Cite this article

Summary:

We evaluated the use of CD34+ selected allogeneic peripheral blood as a source of hematopoietic progenitors for allogeneic transplantation in 11 patients with aplastic anemia (AA). The median age was 17 years (range, 6–49), and the median time between diagnosis and transplant 1 month (range, 1–24). Conditioning consisted of cyclophosphamide (50 mg/kg per day) on days −7 to −4 and antithymocyte globulin (30 mg/kg per day) on days −4 to −2 in nine patients. Total lymphoid irradiation was added to the preparative regimen for two. Graft-versus-host disease (GVHD) prophylaxis consisted of cyclosporine A and prednisone. Median doses of CD34+ and CD3+ cells infused were 3.91 × 106 and 0.3 × 106/kg, respectively. The median time taken to achieve a neutrophil count >0.5 × 109/l was 12 days and to recover a platelet count >20 × 109/l, 13 days. Two patients developed acute GVHD grade I–II and one developed limited chronic GVHD. There were two treatment-related deaths. At a median follow-up of 44 months (range, 4–73), nine patients were alive with sustained and complete engraftment. This is a promising procedure in patients with AA, resulting in a rapid hematopoietic recovery, a low transplant-related mortality, and a low incidence of GVHD.

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Authors and Affiliations

  1. Bone Marrow Transplant Unit, Hematology Service, University Hospital La Fe, Valencia, Spain

    J de la Rubia, S Cantero, G F Sanz, M J Remigia, E Monteagudo, F Moscardó, G Martín, I Lorenzo, C Jiménez, J Martínez, P Montesinos, I Jarque & M A Sanz

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  1. J de la Rubia
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  2. S Cantero
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  3. G F Sanz
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  8. I Lorenzo
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Correspondence to M A Sanz.

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de la Rubia, J., Cantero, S., Sanz, G. et al. Transplantation of CD34+ selected peripheral blood to HLA-identical sibling patients with aplastic anaemia: results from a single institution. Bone Marrow Transplant 36, 325–329 (2005). https://doi.org/10.1038/sj.bmt.1705067

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  • DOI: https://doi.org/10.1038/sj.bmt.1705067

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