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‘Agglutination and flocculation’ of stem cells collected by apheresis due to cryofibrinogen

Bone Marrow Transplantation volume 33, pages 765–767 (2004)Cite this article

Summary:

Collection of peripheral stem cells by apheresis is a well-described process. Here, investigations concerning ‘agglutination and flocculation’ of stem cells collected from two patients are described. In both cases, cryoproteins were observed and cryofibrinogen was identified using high-resolution two-dimensional electrophoresis. In one case, peripheral stem cells were collected after a second course of mobilization, and the cells were immediately washed at 37°C before being frozen, allowing their use, despite the presence of cryofibrinogen. In the other case, ‘agglutination’ was reversed by warming the bag, and plasma was removed before freezing.

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Authors and Affiliations

  1. Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland

    M A Siegenthaler, D-H Vu, D Gasparini & J-D Tissot

  2. Center of Oncology/Hematology and Transfusion Medicine, Kantonsspital Aarau, Aarau, Switzerland

    M Ebnöther, P Schmid & M Bargetzi

  3. Centre Pluridisciplinaire d'Oncologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland

    N Ketterer & F Luthi

Authors
  1. M A Siegenthaler
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  2. D-H Vu
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  3. M Ebnöther
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  4. N Ketterer
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  5. F Luthi
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  6. P Schmid
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  7. M Bargetzi
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  8. D Gasparini
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  9. J-D Tissot
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Correspondence to J-D Tissot.

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Siegenthaler, M., Vu, DH., Ebnöther, M. et al. ‘Agglutination and flocculation’ of stem cells collected by apheresis due to cryofibrinogen. Bone Marrow Transplant 33, 765–767 (2004). https://doi.org/10.1038/sj.bmt.1704420

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  • DOI: https://doi.org/10.1038/sj.bmt.1704420

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