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Dendritic Cells

Use of anti-BDCA-2 antibody for detection of dendritic cells type-2 (DC2) in allogeneic hematopoietic stem cell transplantation

Bone Marrow Transplantation volume 29, pages 887–891 (2002)Cite this article

Abstract

TH2-inducing dendritic cells (DC2) are commonly identified as negative for lineage markers and positive for HLA-DR and CD123 expression. More recently, normal blood DC2 were shown also to be positive for BDCA-2 and BDCA-4 antigens. The aim of this study was to evaluate whether BDCA-2 expression on DC2 is impaired in patients undergoing an allogeneic hematopoietic stem cell transplantation (HSCT) and in healthy donors treated with G-CSF for HSC mobilization. Flow cytometry assays for DC2 detection using either a triple staining with anti-HLA-DR PerCP, anti-Lin+ anti-CD34 FITC and anti-CD123 PE monoclonal antibodies (mAbs), or a double staining with anti-HLA-DR PE and anti-BDCA-2 FITC mAbs were compared in blood samples from patients who underwent an allogeneic HSCT (n = 30) or from healthy donors before (n = 11) and after (n = 8) G-CSF mobilization, as well as in healthy donors’ leukapheresis products (n = 12) or bone marrow (n = 4). Staining of BDCA-2+ cells with other markers such as anti-CD38, anti-CD54 and anti-CD58 were also performed. Median values of CD123+ DC2 and BDCA-2+ DC2 were not statistically different in the blood of patients previously treated with chemotherapy, nor in the blood or bone marrow of heathy donors. Also, a 5 day G-CSF treatment did not affect BDCA-2 or adhesion molecule expression on healthy donors’ blood DC2 significantly. A correlation between all the results (n = 65) obtained with the two assays was demonstrated in a linear regression curve (r = 0.914) (P = 0.00001). BDCA-2 is a marker highly specific for DC2 that is not downregulated by chemotherapy or G-CSF treatment. Therefore, the anti-BDCA-2 mAb can be efficiently combined with other mAbs and used in studies addressing the role of DC2 in the allogeneic HSCT setting.

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References

  1. Cella M, Sallusto F, Lanzavecchia A . Origin, maturation and antigen presenting function of dendritic cells Curr Opin Immunol 1997 9: 10 16

    Article  CAS  Google Scholar 

  2. Rissoan MC, Soumelis V, Kadowaki N et al. Reciprocal control of T helper cell and dendritic cell differentiation Science 1999 283: 1183 1186

    Article  CAS  Google Scholar 

  3. Olweus J, Bitmansour A, Warnke R et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin Proc Natl Acad Sci USA 1997 94: 12551 12556

    Article  CAS  Google Scholar 

  4. Moser M, Murphy KM . Dendritic cell regulation of Th1-Th2 development Nature Immunol 2001 1: 199 205

    Article  Google Scholar 

  5. Grouard G, Rissoan MC, Filgueira L et al. The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligand J Exp Med 1997 185: 1101 1111

    Article  CAS  Google Scholar 

  6. Arpinati M, Green CL, Heimfeld S et al. Granulocyte-colony stimulating factor mobilizes T helper 2-inducing dendritic cells Blood 2000 95: 2484 2490

    CAS  Google Scholar 

  7. Liu YJ, Kanzler H, Soumelis V, Gilliet M . Dendritic cell lineage, plasticity and cross-regulation Nature Immunol 2001 2: 585 589

    Article  CAS  Google Scholar 

  8. Jahnsen FL, Lund-Johansen F, Dunne JF et al. Experimental induced recruitment of plasmacytoid (CD123 high) dendritic cells in human nasal allergy J Immunol 2000 165: 4062 4068

    Article  CAS  Google Scholar 

  9. Liu YJ, Blom B . Introduction: TH2-inducing DC2 for immunotherapy Blood 2000 95: 2482 2483

    CAS  PubMed  Google Scholar 

  10. Locatelli F, Rondelli D, Burgio GR . Tolerance and hematopoietic stem cell transplantation 50 years after Burnet's theory Exp Hematol 2000 28: 479 489

    Article  CAS  Google Scholar 

  11. Dzionek A, Fuchs A, Schmidt P et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood J Immunol 2000 165: 6037 6046

    Article  CAS  Google Scholar 

  12. Lemoli RM, Bandini G, Leopardi G et al. Allogeneic peripheral blood stem cell transplantation in patients with early-phase hematologic malignancy: a retrospective comparison of short-term outcome with bone marrow transplantation Haematologica 1998 83: 48 55

    CAS  Google Scholar 

  13. Shlomchik WD, Couzens MS, Tang CB et al. Prevention of graft versus host disease by inactivation of host antigen-presenting cells Science 1999 285: 412 415

    Article  CAS  Google Scholar 

  14. Matsumoto M, Katoh Y, Nakamura Y et al. Injection of CD4+ and CD8+ cells with donor or host accessory cells induces acute graft-vs-host disease in human skin in immunodeficient mice Exp Hematol 2001 29: 720 727

    Article  CAS  Google Scholar 

  15. Waller EK, Rosenthal H, Jones TW et al. Larger numbers of CD4(bright) dendritic cells in donor bone marrow are associated with increased relapse after allogeneic bone marrow transplantation Blood 2001 97: 2948 2956

    Article  CAS  Google Scholar 

  16. Soumelis V, Scott I, Gheyas F et al. Depletion of circulating natural type 1 interferon-producing cells in HIV-infected AIDS patients Blood 2001 98: 906 912

    Article  CAS  Google Scholar 

  17. Feldman S, Stein D, Amrute S et al. Decreased interferon-alpha production in HIV-infected patients correlates with numerical and functional deficiencies in circulating type 2 dendritic cell precursors Clin Immunol 2001 101: 201 210

    Article  CAS  Google Scholar 

  18. Dzionek A, Sohma Y, Nagafune J et al. BDCA-2, a novel plasmacytoid dendritic cell cell-specific type II C-type lectin, mediates antigen-capture and is a potent inhibitor of interferon-α/β induction J Exp Med 2001 194: 1823 1834

    Article  CAS  Google Scholar 

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Acknowledgements

Anti-BDCA-2 mAb was kindly provided by Dr Juergen Schmitz (Miltenyi Biotec, Bergisch Gladbach, Germany). This research was partly supported by grants from MURST (Rome, Italy), Fondazione CARISBO (Bologna, Italy) and Associazione Italiana contro le Leucemie – Progetto Trenta Ore Per La Vita 2000 (Rome, Italy) to DR.

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  1. Research Center for Transplant Immunology, Institute of Hematology and Medical Oncology ‘Seràgnoli’, University of Bologna, Bologna, Italy

    M Arpinati, G Chirumbolo, B Urbini, V Martelli, M Stanzani, S Falcioni, F Bonifazi, G Bandini, S Tura, M Baccarani & D Rondelli

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Arpinati, M., Chirumbolo, G., Urbini, B. et al. Use of anti-BDCA-2 antibody for detection of dendritic cells type-2 (DC2) in allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant 29, 887–891 (2002). https://doi.org/10.1038/sj.bmt.1703569

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