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Murine Models

Leukaemogenic potency of WEHI-3B cells grown in vitro or in leukaemic mice

Bone Marrow Transplantation volume 28, pages 699–704 (2001)Cite this article

Abstract

In order to quantify contaminating leukaemia inducing cells in blood or bone marrow from WEHI-3B bearing BALB/c mice (injected with 105 WEHI-3B suspension culture cells), in vitro colony forming leukaemia cells (CFU-L) and survival rate and/or survival time of mice transplanted with cells from WEHI bearing mice or suspension culture were correlated. ED50 (inoculum inducing leukaemia in 50% of the animals) was 109 CFU-L (33–361) from suspension culture cells. Three weeks after initiation of leukaemia 2 × 105 BM or 0.5–2.0 × 106 blood cells induced 100% mortality of recipients. After mobilisation with CY or CY and G-CSF, the same amount of blood or BM cells did not induce leukaemia in recipients. A significant negative correlation was found between the survival time of leukaemic mice and the log number of CFU-L inoculated from in vivosources. In terms of CFU-L cells, leukaemia induction to BM or WBC obtained 3 weeks after leukaemia induction were more potent; those from BM or WBC also obtained at 3 weeks but after mobilisation were less potent inducers than those from suspension culture. These data suggest that CFU-L and leukaemogenic cells are associated, but not identical. Bone Marrow Transplantation (2001) 28, 699–704.

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Acknowledgements

We wish to express our thanks to Ms M Renner and Ms O Ullrich for their skilful technical assistance. This work was partly supported by grants 094/2000 and 508/2000 (Hungarian Ministry of Health) and T 026441 (Hungarian Academy of Sciences).

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Authors and Affiliations

  1. National Institute of Haematology and Immunology, Budapest, Hungary

    J Gidáli & I Fehér

  2. Dept of Pharmacology Medical and Health Science Center, University of Debrecen, Hungary

    A Megyeri & P Kovács

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  1. J Gidáli
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  2. I Fehér
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  3. A Megyeri
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Gidáli, J., Fehér, I., Megyeri, A. et al. Leukaemogenic potency of WEHI-3B cells grown in vitro or in leukaemic mice. Bone Marrow Transplant 28, 699–704 (2001). https://doi.org/10.1038/sj.bmt.1703210

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