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Progenitor Cell Mobilisation

Large-scale mobilization and isolation of CD34+ cells from normal donors

Bone Marrow Transplantation volume 26, pages 1271–1279 (2000)Cite this article

Abstract

We describe collection and purification of peripheral blood CD34+ cells from volunteer, normal donors and allogeneic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6 × 1010 which included 1.9 × 108CD34+ cells corresponding to 2.7 × 106 CD34+ cells/kg. The number of CD34+ cells collected did not differ between males and females but did correlate with the donor's weight and the total number of nucleated cells collected. The Nexell Isolex 300i cell separator was used to isolate CD34+ cells from 30 of the collections. A mean of 0.36% of the total cells was recovered and included 43 ± 18% of the CD34+ cells. CD34+ cells represented 85 ± 11% of the recovered cells. The total number of CD34+ cells recovered was not influenced by the number of nucleated cells placed on the Isolex 300i. The percentage of CD34+ cells recovered was not related to the number of CD34+ cells placed on the Isolex 300i. The purity of the final product was influenced by the number of CD34+ cells but not the total number of nucleated cells. An additional 38 CD34+ cell isolations were performed on normal allogeneic stem cell donors with similar results. These observations further support the safety and feasibility of peripheral blood CD34+ cell collection and purification. Bone Marrow Transplantation (2000) 26, 1271–1279.

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Acknowledgements

We would like to thank Nexell, Inc for providing the Isolex 300i columns and AMGEN for providing the G-CSF. Drs Bob Briddell and Ian McNiece provided data on CD34+ counts from apheresis products provided to AMGEN and Elizabeth Cox supplied data on the allogeneic stem cell donors. This work was supported by the Centers of Excellence in Molecular Hematology (NIDDK P30DK49218), National Centers for Research Resources (NIH M01 RR00750) (RA), and the Jonathan and Jennifer Simmons Charitable Trust.

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Authors and Affiliations

  1. Section of Pediatric Hematology/Oncology, Riley Hospital for Children, Indianapolis, IN, USA

    JM Croop, S Kreissman, FO Smith & DA Williams

  2. Herman B Wells Center for Pediatric Research, Riley Hospital for Children, Indianapolis, IN, USA

    R Cooper & DA Williams

  3. Division of Biostatistics, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA

    R Seshadri

  4. Riley Apheresis Center, Indiana University School of Medicine, Indianapolis, IN, USA

    C Fernandez

  5. Department of Pathology, Stem Cell Laboratory, Indiana University School of Medicine, Indianapolis, IN, USA

    V Graves & R Abonour

  6. Division of Hematology/Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA

    K Cornetta & R Abonour

  7. The Howard Hughes Medical Institute, Indiana University School of Medicine, Indianapolis, IN, USA

    DA Williams

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Croop, J., Cooper, R., Seshadri, R. et al. Large-scale mobilization and isolation of CD34+ cells from normal donors. Bone Marrow Transplant 26, 1271–1279 (2000). https://doi.org/10.1038/sj.bmt.1702720

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