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Peripheral Blood Stem Cells

Allogeneic hematopoietic transplantation of CD34+ selected cells from an HLA haplo-identical related donor. A long-term follow-up of 135 patients and a comparison of stem cell source between the bone marrow and the peripheral blood

Bone Marrow Transplantation volume 26, pages 1281–1290 (2000)Cite this article

Abstract

We studied the outcome of allogeneic transplants in 135 patients who received selected BM and/or PBSC CD34+ cells from HLA haplo-identical related donors. Donor engraftment was achieved in 108 of 128 evaluable transplants. Engraftment failure occurred more often in non-malignant than in malignant diseases (10 of 25 vs 17 of 103, P = 0.010). The CD34+ cell dose was associated with the speed of neutrophil and platelet recovery, but the cell source was not. Acute GVHD (⩾grade II) developed in 21.0 ± 3.7%. Chronic GVHD occurred more frequently in malignancies than in non-malignancies (44.1 ± 7.6% vs 0.0%, P = 0.0075), and more in PBSC recipients than in BM recipients (53.6 ± 9.4% vs 17.4 ± 9.3%, P = 0.0054). Relapse rate was higher in high risk patients than in standard risk patients (78.7 ± 7.1% vs 22.1 ± 10.0%, P = 0.0001). Probabilities of disease-free survival (DFS) were 14.2 ± 3.5% in malignancies and 25.7 ± 9.2% in non-malignancies. Probabilities of DFS in standard and high risk patients were 39.4 ± 9.2% and 5.7 ± 2.8% (P = 0.0001). A high incidence of graft failure, infection and relapse was observed and resulted in high mortality. Bone Marrow Transplantation (2000) 26, 1281–1290.

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Acknowledgements

This work was supported in part by the Grant-in-Aid for Cancer Research (10-1) from the Ministry of Health and Welfare of Japan. The authors thank Mr Toshiaki Tanaka, Dr Adam Tucker, Dr Yasuo Haruki and Ms Mari Sato for their assistance in statistical analysis and preparation of the manuscript.

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Authors and Affiliations

  1. Department of Pediatrics, Tokai University School of Medicine, Isehara, Japan

    S Kato & H Yabe

  2. Department of Pediatrics, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan

    M Yasui & K Kawa

  3. Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Japan

    T Yoshida

  4. Department of Pediatrics, University of Akita School of Medicine, Akita, Japan

    A Watanabe

  5. Department of Developmental Medicine, D-5, Osaka University Graduate School of Medicine, Osaka, Japan

    Y Osugi

  6. Department of Pediatrics, Nagoya University School of Medicine, Nagoya, Japan

    K Horibe

  7. Department of Internal Medicine, Japanese Red Cross Nagoya First Hospital, Nagoya, Japan

    Y Kodera

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Kato, S., Yabe, H., Yasui, M. et al. Allogeneic hematopoietic transplantation of CD34+ selected cells from an HLA haplo-identical related donor. A long-term follow-up of 135 patients and a comparison of stem cell source between the bone marrow and the peripheral blood. Bone Marrow Transplant 26, 1281–1290 (2000). https://doi.org/10.1038/sj.bmt.1702707

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