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Murine Models

Prevention of autoimmune hearing loss in MRL/lpr mice by bone marrow transplantation

Bone Marrow Transplantation volume 26, pages 887–892 (2000)Cite this article

Abstract

We examined the effects of bone marrow transplantation (BMT) on immune-mediated inner ear diseases in MRL/Mp-lpr/lpr (MRL/lpr) mice, which manifest not only lupus nephritis but also sensorineural hearing loss (SNHL) at the age of 20 weeks. These mice were treated with cyclophosphamide (CY) and irradiation (5 Gy × 2), followed by the transplantation of bones plus bone marrow cells from allogeneic normal C57BL/6 mice at the age of 12 weeks. Hematolymphoid cells were reconstituted with donor-derived cells 3 months after BMT. Thus-treated MRL/lpr mice showed neither lupus nephritis nor SNHL even 24 weeks after BMT. No pathological findings were observed in either glomeruli or cochleae. These findings suggest that BMT can be used to prevent the development of autoimmune SNHL in MRL/lpr mice. Bone Marrow Transplantation (2000) 26, 887–892.

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Acknowledgements

We thank Ms K Ando and Mr H Eastwick-Field for their help in the preparation of the manuscript. This work was supported by a grant application agency (grants-in-aid for scientific research 08671994 and 11671712 from the Ministry of Education, Science and Culture).

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Kansai Medical University, Osaka, Japan

    H Iwai & T Yamashita

  2. First Department of Pathology, Kansai Medical University, Osaka, Japan

    S Lee, K Sugiura, M Inaba & S Ikehara

  3. Department of Orthopedic Surgery, Kansai Medical University, Osaka, Japan

    K Takeuchi & T Kushida

  4. Department of Otorhinolaryngology, Kanazawa Medical University, Ishikawa, Japan

    K Tomoda

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  1. S Lee
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  2. H Iwai
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Lee, S., Iwai, H., Sugiura, K. et al. Prevention of autoimmune hearing loss in MRL/lpr mice by bone marrow transplantation. Bone Marrow Transplant 26, 887–892 (2000). https://doi.org/10.1038/sj.bmt.1702636

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  • DOI: https://doi.org/10.1038/sj.bmt.1702636

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