Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Graft-Versus-Host Disease

Sequential analysis of HLA-C-specific killer cell inhibitory receptor (CD158b) expressing peripheral blood mononuclear cells during chronic graft-versus-host disease

Bone Marrow Transplantation volume 26, pages 287–290 (2000)Cite this article

Abstract

We have sequentially investigated the expression of natural killer cell inhibitory receptors (KIRs) for HLA-C (CD158b) on peripheral blood mononuclear cells (PBMC) in three patients with extensive chronic graft-versus-host disease (cGVHD) after allogeneic bone marrow transplantation (alloBMT). Clinical symptoms of cGVHD were not cured and worsened in the first patient whose CD158b-positive cells increased to 18.5% during cGVHD and decreased to 9.4% at 8 months after transplantation. On the other hand, cGVHD was cured and did not relapse in the second patient whose CD158b-positive cells increased up to 45.9% during cGVHD and sustained 19.4% at 8 months after transplantation. In contrast, CD158b-positive cells were less than 10% during the course of cGVHD in the third patient, and her cGVHD did not respond to treatment. Therefore, it appears that chronic allostimulation augments the expansion of CD158b-positive cells and these expanded CD158b-positive cells may have some role in the control of alloresponse in some patients. Bone Marrow Transplantation (2000) 26, 287–290.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

Similar content being viewed by others

References

  1. Moretta A, Biassoni R, Bottino C et al. Major histocompatibility complex class I-specific receptors on human natural killer and T lymphocytes Immunol Rev 1997 155: 105–117

    Article  CAS  Google Scholar 

  2. Biassoni R, Falco M, Cambiaggi A et al. Amino acid substitutions can influence the NK-mediated recognition of HLA-C molecules. Role of Serine-77 and Lysine-80 in the target cell protection from lysis mediated by ‘Group 2’ or ‘Group 1’ NK clones J Exp Med 1995 182: 605–609

    Article  CAS  Google Scholar 

  3. Phillips JH, Gumperz JE, Parham P, Lanier LL . Superantigen-dependent, cell-mediated cytotoxicity inhibited by MHC class I receptors on T lymphocytes Science 1995 268: 403–405

    Article  CAS  Google Scholar 

  4. Mingari MC, Moretta A, Moretta L . Regulation of KIR expression in human T cells: a safe mechanism that may impair protective T-cell responses Immunol Today 1998 19: 153–157

    Article  CAS  Google Scholar 

  5. Leibson PJ . MHC-recognizing receptors: they're not just for T-cells anymore Immunity 1995 3: 5–8

    Article  CAS  Google Scholar 

  6. Albi N, Ruggeri L, Aversa F et al. Natural killer (NK)-cell function and antileukemic activity of a large population of CD3+/CD8+ T cells expressing NK receptors for major histocompatibility complex class I after ‘Three-Loci’ HLA-incompatible bone marrow transplantation Blood 1996 87: 3993–4000

    CAS  PubMed  Google Scholar 

  7. Cambiaggi A, Verthuy C, Naquet P et al. Natural killer cell acceptance of H-2 mismatch bone marrow grafts in transgenic mice expressing HLA-Cw3 specific killer cell inhibitory receptor (CD158b) Proc Natl Acad Sci USA 1997 94: 8088–8092

    Article  CAS  Google Scholar 

  8. Ruggeri L, Capanni M, Casucci M et al. Role of natural killer cell alloreactivity in HLA-mismatched hematopoietic stem cell transplantation Blood 1999 94: 333–339

    CAS  Google Scholar 

  9. Cambiaggi A, Darche S, Guia S et al. Modulation of T-cell functions in KIR2DL3 (CD158b) transgenic mice Blood 1999 94: 2396–2402

    CAS  PubMed  Google Scholar 

  10. Tanaka J, Mori A, Ohta S et al. Expression of HLA-C specific natural killer cell receptor (CD158a and CD158b) on peripheral blood mononuclear cells after allogeneic bone marrow transplantation Br J Haematol 2000 108: 778–783

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Ms Yamane for her technical assistance. This work was supported in part by a grant from Japanese Foundation for Multidisciplinary Treatment of Cancer and Bone Marrow Transplantation Research Committee of the Ministry of Health and Welfare of Japan and Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.

Author information

Authors and Affiliations

  1. Department of Hematology and Oncology, Hokkaido University School of Medicine, Sapporo, Japan

    J Tanaka, Y Tutumi & M Imamura

  2. Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan

    A Mori, S Ohta, S Kobayashi & M Asaka

Authors
  1. J Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y Tutumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S Ohta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M Asaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M Imamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanaka, J., Tutumi, Y., Mori, A. et al. Sequential analysis of HLA-C-specific killer cell inhibitory receptor (CD158b) expressing peripheral blood mononuclear cells during chronic graft-versus-host disease. Bone Marrow Transplant 26, 287–290 (2000). https://doi.org/10.1038/sj.bmt.1702511

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.bmt.1702511

Keywords

This article is cited by

Search

Advanced search

Quick links