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CD34+ selection of hematopoietic blood cell collections and autotransplantation in lymphoma: overnight storage of cells at 4°C does not affect outcome

Bone Marrow Transplantation volume 25, pages 559–566 (2000)Cite this article

Abstract

The purpose of this study was to investigate whether storing mobilized peripheral blood progenitor cell (PBPC) collections overnight before CD34+ selection may delay platelet count recovery after high-dose chemotherapy and CD34+-enriched PBPC re-infusion. Lymphoma patients underwent PBPC mobilization with cyclophosphamide 4 g/m2 i.v. and G-CSF 10 μg/kg/day subcutaneously. Patients were prospectively randomized to have each PBPC collection enriched for CD34+ cells with the CellPro CEPRATE SC System either immediately or after overnight storage at 4°C. Thirty-four patients were randomized to overnight storage and 34 to immediate processing of PBPC; 15 were excluded from analysis due to tumor progression or inadequate CD34+ cell mobilization. PBPC from 23 patients were stored overnight, while 30 subjects underwent immediate CD34+selection and cryopreservation. Median yield of CD34+ enrichment was 43.6% in the immediate processing group compared to 39.1% in the overnight storage group (P = 0.339). neutrophil recovery >500 × 109/l occurred a median of 11 days (range 9–16 days) in the overnight storage group compared to 10.5 days (range 9–21 days) in the immediate processing group (P = 0.421). Median day to platelet transfusion independence was 13 (range 7–43) days in the overnight storage group vs 13.5 (range 8–35) days in those assigned to immediate processing (P = 0.933). We conclude that storage of PBPC overnight at 4°C allows pooling of consecutive-day collections resulting in decreased costs and processing time without compromising neutrophil and platelet engraftment after infusion of CD34+-selected progenitor cells. Bone Marrow Transplantation (2000) 25, 559–566.

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Acknowledgements

We thank Howard J Meyerson, MD, for flow cytometric study assistance, and Donna Speron, Lisa McDonnel, and Cindy Jacobs, PhD, MD for their expert support in the conduct of this protocol and in the preparation of the manuscript. This work was supported, in part, by a grant from CellPro, Inc., Bothell, WA, and Public Health Service grants M01RR00080, P30CA43703, and P30CA16086 from the National Institutes of Health, the National Cancer Institute and the Department of Health and Human Services.

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Authors and Affiliations

  1. Department of Medicine, Ireland Cancer Center of Case Western Reserve University, University Hospitals of Cleveland, Cleveland, OH, USA

    HM Lazarus, ON Koç, BW Cooper & SL Gerson

  2. Hackensack Medical Center, Hackensack, NJ, USA

    AL Pecora

  3. University of North Carolina, Chapel Hill, NC, USA

    TC Shea & DA Gabriel

  4. CellPro, Inc, Bothell, WA, USA

    JM White, M Krieger & AP Sing

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  1. HM Lazarus
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Lazarus, H., Pecora, A., Shea, T. et al. CD34+ selection of hematopoietic blood cell collections and autotransplantation in lymphoma: overnight storage of cells at 4°C does not affect outcome. Bone Marrow Transplant 25, 559–566 (2000). https://doi.org/10.1038/sj.bmt.1702175

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