Abstract
PAPER chromatography of the p-nitrobenzene-azodimethoxyaniline (‘Fast Black Salt K’) derivatives of two commercial samples of œstrone, according to the method described by Heftmann1, revealed the presence of small traces of an impurity which appeared as a blue spot with lower RF value than the pink œstrone dye. This substance could be removed from the œstrone by means of partition chromatography on a ‘Celite’–sodium hydroxide column, following the method of Swyer and Braunsberg2. Paper chromatograms, run before and after purification by our technique, showed that the impurity was completely removed by this means (see photograph).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Heftmann, E., Science, 111, 571 (1950); J. Amer. Chem. Soc., 73, 851 (1951).
Swyer, G. I. M., and Braunsberg, H., J. Endocrin., 7, lx (1951).
Mentioned by Carol, J., J. Amer. Pharm. Assoc., Sci. Ed., 39, 425 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BRAUNSBERG, H. Purification of Commercial Samples of Œstrone. Nature 169, 967–968 (1952). https://doi.org/10.1038/169967b0
Issue Date:
DOI: https://doi.org/10.1038/169967b0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.