Abstract
IN a recent report elsewhere1 we have shown that the œstrone and œstradiol in the phenolic fraction of urinary extracts can be separated satisfactorily, in microgram quantities, by means of a silica column, using aqueous sodium hydroxide as the stationary phase and benzene as the moving phase. We were at that time unable to deal with the œstriol fraction chromatographically, and separated it chemically by the method of Bachman and Petit2; this, however, was a tedious process and left unsolved the problem of purification so essential for satisfactory determination by fluorimetry using phosphoric acid. We have now succeeded in overcoming both these difficulties in one operation, and can separate the three fractions from the crude phenolic extract, achieving a satisfactory degree of purification in the process, on a single silica column.
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References
Swyer, G. I. M., and Braunsberg, H., J. Endocrinol., 7, lx (1951).
Bachman, C., and Petit, D. S., J. Biol. Chem., 138, 689 (1941).
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STERN, M., SWYER, G. Separation of Urinary Œstrogens by Micro-Partition Chromatography. Nature 169, 796 (1952). https://doi.org/10.1038/169796a0
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DOI: https://doi.org/10.1038/169796a0
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Beitrag zur Chromatographie von Oestrogenen
The Science of Nature (1955)
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