Abstract
ATTEMPTS have been made to measure the total concentration of fixed base (sodium, potassium, calcium and magnesium) in the cells of the renal cortex of adult male rats under different conditions. All experiments were carried out with thin slices prepared as for manometric work, and the total concentration of base was determined in portions of moist tissue weighing 300–500 mgm. by the benzidine sulphate method described by Peters and van Slyke1 for serum. The media used in all these experiments were as described by Robinson2, and slices which were respiring were maintained at 38° C. in oxygenated solutions in the apparatus described for the “Short Experiments” in the same publication2.
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References
Peters, J. P., and van Slyke, D. D., “Quantitative Clinical Chemistry”, 2, “Methods” (London: Baillière, Tindall and Cox, 1932).
Robinson, J. R., Proc. Roy. Soc., B, 137, 378 (1950).
McCance, R. A., and Widdowson, E. M., “The Chemical Composition of Foods”. M.R.C. Spec. Rep. 235, 2nd edit. (H.M.S.O., London, 1946).
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ROBINSON, J. Total Concentration of Fixed Base in Cells of the Renal Cortex of the Rat. Nature 169, 713–714 (1952). https://doi.org/10.1038/169713a0
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DOI: https://doi.org/10.1038/169713a0
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