Abstract
THE resolution of a mixture of corticosteroids into its components by partition chromatography on cellulose columns necessitates the handling of a large number of fractions, owing to the widely differing RF values of the fastest and the slowest of these compounds. Thus a column made up of 3.5 gm. of powdered cellulose may require as much as 1,000 ml. of solvent (toluene/propylene glycol) to elute compound F. It is therefore desirable, prior to the working up of the individual fractions, to have an idea of their likely steroid content and to eliminate those not containing any active material.
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References
Nelson, N., J. Biol. Chem., 153, 375 (1944).
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SCHWARZ, V. A Rapid Semi-quantitative Spot Test for Reducing Steroids. Nature 169, 506–507 (1952). https://doi.org/10.1038/169506b0
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DOI: https://doi.org/10.1038/169506b0
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