Abstract
VARIOUS methods for spreading proteins have been devised. The method used by Gorter and Grendel1 is to drop a fairly concentrated aqueous protein solution very carefully from the least possible height on to the clean water surface in the trough. Hughes et al.2 found that solid particles of protein spread very rapidly on a clean substrate surface. These methods proved to be unsuccessful for the spreading of arachin. Arachin was also not soluble in the aqueous propyl alcohol–sodium acetate mixture recommended by Ställberg and Teorell3. Very satisfactory monolayers of egg albumin and β-lacto-globulin were obtained by Bull4 by using 35 per cent ammonium sulphate as the underlying solution. Accordingly, arachin was next spread on strong ammonium sulphate solutions, and films stable up to high film pressures were obtained. The ammonium sulphate was of analytical quality and the solutions were treated with activated carbon black to remove all surface-active impurities.
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References
Gorter, E., and Grendel, F., Proc. K. Akad. Amsterdam, 29, 371 (1925).
Hughes, A. H., Schulman, J. H., and Rideal, E. K., Nature, 129, 21 (1932).
Ställberg, S., and Teorell, T., Trans. Farad. Soc., 35, 1413 (1939).
Bull, H. B., J. Amer. Chem. Soc., 67, 10 (1945).
Bull, H. B., “Advances in Protein Chem.”, 3, 95 (1947).
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Johnson, P., Trans. Farad. Soc., 42, 36 (1946).
Johnson, P., and Joubert, F. J., J. Polymer Sci., 7, 605 (1951).
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JOUBERT, F. Monolayers of the Globulin, Arachin. Nature 169, 498 (1952). https://doi.org/10.1038/169498a0
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DOI: https://doi.org/10.1038/169498a0
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