Abstract
IN recent methods1 for the isolation of deoxyribonucleic acids from mammalian tissues, the use of buffered (pH 7) solvents containing low concentrations of sodium arsenate, sodium citrate or sodium borate has been advocated in order to prevent enzymicdepolymerization during the initial extraction of the deoxyribonucleohistone. As these salts are known to inhibit the action of pancreas deoxyribonuclease2, their use in this connexion presumably rests upon the tacit assumption that the intracellular deoxypentosenucleases of mammalian tissues3 have properties similar to those of the pancreas enzyme.
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WEBB, M. Use of Deoxyribonuclease Inhibitors in the Isolation of Deoxyribonucleic Acids. Nature 169, 417 (1952). https://doi.org/10.1038/169417a0
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DOI: https://doi.org/10.1038/169417a0
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