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Diphosphopyridine Nucleotide and Phosphate Requirement for Oxidation of Amino-Acids by Cell-free Extracts of Obligate Anaerobes

Nature volume 169, pages 243–244 (1952)Cite this article

Abstract

PREVIOUS studies have shown that resting cells of Clostridia (Cl. sporogenes GO1, and Cl. saccharobutyricum GR4) may bring about the oxidative deamination of certain amino-acids in the presence of oxygen as hydrogen acceptor1,2. In order to study in greater detail the mechanism of the L-amino-acid oxidation, cell-free extracts of these bacteria were prepared by using ultrasonic vibration, at 960 kc./s., 11.3 W./cm.2, in presence of a large excess of catalase.

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Authors and Affiliations

  1. Laboratoire de chimie bactérienne, Institut Pasteur, Garches, Seine et Oise

    B. NISMAN & J. MAGER

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  1. B. NISMAN
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  2. J. MAGER
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NISMAN, B., MAGER, J. Diphosphopyridine Nucleotide and Phosphate Requirement for Oxidation of Amino-Acids by Cell-free Extracts of Obligate Anaerobes. Nature 169, 243–244 (1952). https://doi.org/10.1038/169243a0

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